Cyclic GMP binding activity in Dictyostelium discoideum

Identification of a nucleic acid-regulated cyclic GMP-binding activity in Dictyostelium discoideum

Using ion-exchange chromatography, we have identified and isolated two forms of a cyclic GMPspecific binding activity in filter-broken cell extracts of Dictyostelium discoideum. Upon addition of excess cold ligand, one form (S-type) released bound H-labelled cyclic GMP very slowly (fj ~ 68 min), while the other form (F-type) released the cyclic GMP in <1 min. After photoaffinity labelling with ...

Nonequilibrium kinetics of a cyclic GMP-binding protein in dictyostelium discoideum

Chemoattractants added to cells of the cellular slime mold dictyostelium discoideum induce a transient elevation of cyclic GMP levels, with a maximum at 10 s and a recovery of basal levels at approximately 25 s after stimulation. We analyzed the kinetics of an intracellular cGMP binding protein in vitro and in vivo. The cyclic GMP binding protein in vitro at 0 degrees C can be described by its ...

3':5'-cyclic AMP-dependent 3':5'-cyclic GMP accumulation in Dictyostelium discoideum.

Suspensions of 3':5'-cyclic AMP (cAMP)-sensitive cells of Dictyostelium discoideum responded to a cAMP pulse with increased 3':5'-cyclic GMP (cGMP) levels. Under the assay conditions used (2 x 10(8) cells per ml in 10 mM phosphate buffer, pH 6.0) cAMP (5 x 10(-8) M final concentration) increased cGMP levels from 1 pmol per 10(7) cells to 7 pmol per 10(7) cells in 10 sec and basal levels were re...

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum.

TMB-8 inhibits respiration and cyclic GMP formation in Dictyostelium discoideum.

The putative inhibitor of intracellular calcium mobilization, TMB-8 was found to be a powerful inhibitor of respiration in amoebae of Dictyostelium discoideum. Consequently, the previously reported effects of this drug on cyclic GMP formation induced by chemoattractants were reassessed. It was found that TMB-8 abolished both folate and cyclic AMP-mediated accumulation of cyclic GMP in D. discoi...